IL17F Antibody
IL-17F antibody
Product Specifications
Product Name Alternative
Mouse anti-IL-17F antibody, mouse anti-Interleukin-17F antibody, Cytokine ML-1, Interleukin-24
UniProt
Q96PD4
Reactivity
Human
Immunogen
Anti-IL-17F (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-17F produced in E.coli followed by hybridoma development.
Target
IL17F
Clonality
Monoclonal
Clone
4H450.1
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Concentration
1.0 mg/mL
Dilution
ELISA: 1:10,000 - 1:50,000, WB: 0.2μg/mL
Purity
Anti-IL-17F was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human IL-17F protein. A BLAST analysis was used to suggest cross-reactivity with IL-17F from human sources based on 100% homology with the immunizing sequence. Cross-reactivity with IL-17F from other sources has not been determined.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-IL-17F antibody has been tested for use in Western Blot. Specific conditions for reactivity should be optimized by the end user.
Tested Applications
ELISA, WB
NCBI Accession Number
NP_443104.1
Host or Source
Mouse
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG1
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