BmpA, BB_0383 Antibody

This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi p39 protein. A BLAST analysis was used to suggest cross-reactivity with p39 from B. burgdorferi and B. afzelii sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against B. garinii sources based on 88-95% homology and to B.spielmanii A14S based on 86-96% homology. Cross-reactivity with p39 from other sources has not been determined.

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This protein-A purified antibody has been tested for use in ELISA and Western blotting. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 35.4 kDa in size corresponding to Borrelia burgdorferi p39 protein by Western blotting in the appropriate cell lysate or extract.