POFUT1 Antibody (C-term) [APR12919G]
Product Specifications
Background
Tissue Location: Highly expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas
Synonyms
GDP-fucose protein O-fucosyltransferase 1, Peptide-O-fucosyltransferase 1, O-FucT-1, POFUT1, FUT12, KIAA0180
Gene Name
POFUT1
Gene ID
23509
UniProt
Q9H488
Chromosomal Location
Endoplasmic reticulum {ECO:0000250|UniProtKB:Q6EV70}
Applications
WB, IHC-P
Dilution
WB--1:1000 IHC-P--1:100~500
Form
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Function
Catalyzes the reaction that attaches fucose through an O- glycosidic linkage to a conserved serine or threonine residue found in the consensus sequence C2-X (4,5) -[S/T]-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines. Specifically uses GDP- fucose as donor substrate and proper disulfide pairing of the substrate EGF domains is required for fucose transfer. Plays a crucial role in NOTCH signaling. Initial fucosylation of NOTCH by POFUT1 generates a substrate for FRINGE/RFNG, an acetylglucosaminyltransferase that can then extend the fucosylation on the NOTCH EGF repeats. This extended fucosylation is required for optimal ligand binding and canonical NOTCH signaling induced by DLL1 or JAGGED1. Fucosylates AGRN and determines its ability to cluster acetylcholine receptors (AChRs) .
Molecular Weight
43956
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Overview
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality POFUT1 Antibody (C-term) [APR12919G].
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/?term=23509
Uniprot URL
https://www.uniprot.org/uniprot/Q9H488
Available Sizes
Curated Selection
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