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Anti-Ms CD86 Purified

CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152) . The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.

Product Specifications

Certification

RUO

Reactivity

Mouse

Immunogen

LPS-activated CBA/Cs mouse splenic B cells

Target Antigen

CD86

Clone

GL-1

Applications

FC (QC tested), IP, IHC-F, ICC

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Steptoe RJ, Ritchie JM, Jones LK, Harrison LC: Autoimmune diabetes is suppressed by transfer of proinsulin-encoding Gr-1+ myeloid progenitor cells that differentiate in vivo into resting dendritic cells. Diabetes. 2005 Feb;54 (2) :434-42., URL: https://www.ncbi.nlm.nih.gov/pubmed/15677501?itool, *Nolan A, Kobayashi H, Naveed B, Kelly A, Hoshino Y, Hoshino S, Karulf MR, Rom WN, Weiden MD, Gold JA: Differential role for CD80 and CD86 in the regulation of the innate immune response in murine polymicrobial sepsis. PLoS One. 2009 Aug 12;4 (8) :e6600., URL: https://www.ncbi.nlm.nih.gov/pubmed/19672303?itool, *Brasel K, De Smedt T, Smith JL, Maliszewski CR: Generation of murine dendritic cells from flt3-ligand-supplemented bone marrow cultures. Blood. 2000 Nov 1;96 (9) :3029-39., URL: https://www.ncbi.nlm.nih.gov/pubmed/11049981?itool, *Nolan A, Weiden M, Kelly A, Hoshino Y, Hoshino S, Mehta N, Gold JA: CD40 and CD80/86 act synergistically to regulate inflammation and mortality in polymicrobial sepsis. Am J Respir Crit Care Med. 2008 Feb 1;177 (3) :301-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/17989345?itool, *Edgtton KL, Kausman JY, Li M, O'Sullivan K, Lo C, Hutchinson P, Yagita H, Holdsworth SR, Kitching AR: Intrarenal antigens activate CD4+ cells via co-stimulatory signals from dendritic cells. J Am Soc Nephrol. 2008 Mar;19 (3) :515-26., URL: https://www.ncbi.nlm.nih.gov/pubmed/18184859?itool, *Hathcock KS, Laszlo G, Dickler HB, Bradshaw J, Linsley P, Hodes RJ: Identification of an alternative CTLA-4 ligand costimulatory for T cell activation. Science. 1993 Nov 5;262 (5135) :905-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/7694361?itool=, *Benschop RJ, Melamed D, Nemazee D, Cambier JC: Distinct signal thresholds for the unique antigen receptor-linked gene expression programs in mature and immature B cells. J Exp Med. 1999 Sep 20;190 (6) :749-56., URL: https://www.ncbi.nlm.nih.gov/pubmed/10499913?itool, *Radhakrishnan S, Arneson LN, Upshaw JL, Howe CL, Felts SJ, Colonna M, Leibson PJ, Rodriguez M, Pease LR: TREM-2 mediated signaling induces antigen uptake and retention in mature myeloid dendritic cells. J Immunol. 2008 Dec 1;181 (11) :7863-72., URL: https://www.ncbi.nlm.nih.gov/pubmed/19017976?itool, *Chung JB, Wells AD, Adler S, Jacob A, Turka LA, Monroe JG: Incomplete activation of CD4 T cells by antigen-presenting transitional immature B cells: implications for peripheral B and T cell responsiveness. J Immunol. 2003 Aug 15;171 (4) :1758-67., URL: https://www.ncbi.nlm.nih.gov/pubmed/12902475?itool

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The rat monoclonal antibody GL-1 reacts with an extracellular epitope of CD86 (B7-2), a 70-80 kDa type I transmembrane glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.

Applications Notes

Flow cytometry: Recommended dilution: 2 μg/ml; positive control: murine splenocytes.

Isotype

Rat IgG2a kappa

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