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Anti-Cytokeratin 10 Purified

Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

Cytoskeleton preparation extracted from human epidermis by detergent/high salt extraction.

Target Antigen

Cytokeratin 10

Clone

VIK-10

Applications

IHC-P, IP, ICC

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Ivanyi D, Minke JM, Hageman C, Groeneveld E, van Doornewaard G: Patterns of expression of feline cytokeratins in healthy epithelia and mammary carcinoma cells. Am J Vet Res. 1992 Mar;53 (3) :304-14., URL: https://pubmed.ncbi.nlm.nih.gov/7690208/

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The antibody VIK-10 reacts with cytokeratin 10 (56.5 kDa; intracellular antigen) . Cytokeratins are members of intermediate filaments subfamily represented in epithelial tissues.

Applications Notes

Immunohistochemistry (paraffin sections) : Recommended dilution: 10 μg/ml; positive tissue: skin, pretreatment: trypsin/temperature 37 °C.

Isotype

Mouse IgG1

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