Flubi-2

Flubi-2 is a fluorescein-biotin based pH-ratio dye (Ex=480 nm, Em= 520-560 nm) with a pK value of 6.7. Flubi-2 is a hydrolysis product of Flubida-2 (non-fluorescent, membrane permeable). Flubi-2 can be used for pH determination of organelles of the secretory pathway (such as golgi apparatus and endoplasmic reticulum) in living cells[1][2].

O=C1C=CC(C(OC2=CC(O)=CC=C23)=C1)=C3C4=CC=C(C(N(C)CCCCCCN(C)C(CCCC[C@@]5([H])C(NC(N6)=O)C6CS5)=O)=O)C=C4C(O)=O.O=C7C=CC(C(OC8=CC(O)=CC=C89)=C7)=C9C%10=CC(C(N(C)CCCCCCN(C)C(CCCC[C@@]%11([H])C(NC(N%12)=O)C%12CS%11)=O)=O)=CC=C%10C(O)=O

[1]Wu MM, et al. Studying organelle physiology with fusion protein-targeted avidin and fluorescent biotin conjugates. Methods Enzymol. 2000;327:546-64. |[2]Wu MM, et al. Organelle pH studies using targeted avidin and fluorescein-biotin. Chem Biol. 2000 Mar;7(3):197-209.