IGFBP3 Antibody (Biotin)
IGFBP3 Antibody (Biotin)
Product Specifications
Product Name Alternative
IBP3, BP-53, IBP3, Insulin-like growth factor-binding protein 3IBP-3
UniProt
P17936
Reactivity
Human
Immunogen
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIGF-BP3. Human IGF-BP3 specific antibody was purified by affinity chromatography and then biotinylated.
Target
IGFBP3
Clonality
Polyclonal
Conjugation
Biotin
Field of Research
Cell Biology
Concentration
Batch dependent
Form
Lyophilized
Storage Conditions
IGF-BP3 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA:Sandwich:To detect hIGF-BP3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human IGF-BP3 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP3. Western Blot:To detect hIGF-BP3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Tested Applications
ELISA, WB
NCBI Accession Number
P17936
Host or Source
Rabbit
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