Bafilomycin A1

Bafilomycin A1 (BafA1) is a specific and reversible inhibitor of vacuolar H+-ATPase (V-ATPase) with IC50 values of 4-400 nmol/mg. Bafilomycin A1, a macrolide antibiotic, is also used as an autophagy inhibitor at the late stage. Bafilomycin A1 blocks autophagosome-lysosome fusion and inhibits acidification and protein degradation in lysosomes of cultured cells. Bafilomycin A1 induces apoptosis[1][2][3].

Product Specifications

CAS Number

[88899-55-2]

Product Name Alternative

BafA1

UNSPSC

12352005

Hazard Statement

H227, H315, H319, H335

Target

Antibiotic; Apoptosis; Autophagy; Bacterial; Proton Pump

Type

Natural Products

Related Pathways

Anti-infection; Apoptosis; Autophagy; Membrane Transporter/Ion Channel

Applications

Cancer-programmed cell death

Field of Research

Cancer; Infection

Assay Protocol

https://www.medchemexpress.com/Bafilomycin_A1.html

Purity

99.95

Solubility

DMSO : ≥ 100 mg/mL

Smiles

C[C@H]([C@](O[C@@H]1C(C)C)(C[C@@H](O)[C@@H]1C)O)[C@H](O)[C@@H]([C@](OC(/C(OC)=C/C(C)=C\[C@@H](C)[C@@H](O)[C@@H](C)C2)=O)([H])[C@H](/C=C/C=C2\C)OC)C

Molecular Formula

C35H58O9

Molecular Weight

622.83

Precautions

H227, H315, H319, H335

References & Citations

[1]Yuan N, et al. Bafilomycin A1 targets both autophagy and apoptosis pathways in pediatric B-cell acute lymphoblastic leukemia. Haematologica. 2015 Mar;100 (3) :345-56.|[2]Lu X, et al. Bafilomycin A1 inhibits the growth and metastatic potential of the BEL-7402 liver cancer and HO-8910 ovarian cancer cell lines and induces alterations in their microRNA expression. Exp Ther Med. 2015 Nov;10 (5) :1829-1834.|[3]Ohkuma S, et al. Inhibition of cell growth by bafilomycin A1, a selective inhibitor of vacuolar H (+) -ATPase. In Vitro Cell Dev Biol Anim. 1993 Nov;29A (11) :862-6.|[4]Ohta T, et al. Bafilomycin A1 induces apoptosis in the human pancreatic cancer cell line Capan-1. J Pathol. 1998 Jul;185 (3) :324-30.|[5]Mauvezin C, et al. Bafilomycin A1 disrupts autophagic flux by inhibiting both V-ATPase-dependent acidification and Ca-P60A/SERCA-dependent autophagosome-lysosome fusion. Autophagy. 2015;11 (8) :1437-1438.|[6]Yuan N, et al. Bafilomycin A1 targets both autophagy and apoptosis pathways in pediatric B-cell acute lymphoblastic leukemia. Haematologica. 2015;100 (3) :345-356.|[7]Yoshimori T, et al. Bafilomycin A1, a specific inhibitor of vacuolar-type H (+) -ATPase, inhibits acidification and protein degradation in lysosomes of cultured cells. J Biol Chem. 1991;266 (26) :17707-17712|[8]Bowman EJ, et al. Bafilomycins: a class of inhibitors of membrane ATPases from microorganisms, animal cells, and plant cells. Proc Natl Acad Sci U S A. 1988;85 (21) :7972-7976.|[9]Cattani L, et al. Bafilomycin A1 and intracellular multiplication of Legionella pneumophila. Antimicrob Agents Chemother. 1997;41 (1) :212-214.|[10]Yuan N, et al. Bafilomycin A1 targets both autophagy and apoptosis pathways in pediatric B-cell acute lymphoblastic leukemia. Haematologica. 2015 Mar;100 (3) :345-56.