NOV Antibody
NOV Antibody
Product Specifications
Product Name Alternative
CCN3, NOVh, IBP-9, IGFBP9, IGFBP-9, CCN3, NOVH, Protein NOV homolog, CCN family member 3, NovH
UniProt
P48745
Reactivity
Human
Immunogen
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hNOV. Human NOV specific antibody was purified by affinity chromatography employing immobilized hNOV matrix.
Target
NOV
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
Batch dependent
Form
Lyophilized
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA:Indirect:To detect hNOV by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNOV.SandwichTo detect hNOV by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human NOV as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNOV. Western Blot:To detect hNOV by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNOV is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Tested Applications
ELISA, WB
NCBI Accession Number
P48745
Host or Source
Rabbit
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