PIWIL1 antibody
Product Specifications
Background
Plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Directly binds methylated piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Probable component of some RISC complex, which mediates RNA cleavage and translational silencing. Also plays a role in the formation of chromatoid bodies and is required for some miRNAs stability (By similarity) . Isoform 3 may be a negative developmental regulator (PubMed:12037681, PubMed:16287078) .
Synonyms
Piwi-like protein 1|PIWIL1|HIWI
Gene ID
9271
UniProt
Q96J94
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
Piwi-like 1 (Drosophila)
Target
PIWIL1
Clonality
Polyclonal
Isotype
IgG
Applications
ELISA, WB, IF, IHC, IP
Field of Research
Metabolism, Developmental biology
Purification
Immunogen affinity purified
Dilution
WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200; IF: 1:20-1:200
Purity
≥95% as determined by SDS-PAGE
Form
Liquid
Molecular Weight
85-95 kDa
Shipping Conditions
4°C with ice bag
Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)
Tested Applications
ELISA, WB, IF, IHC, IP
Curated Selection
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