ATR antibody
Product Specifications
Background
Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication.
Synonyms
Serine/threonine-protein kinase ATR|Ataxia telangiectasia and Rad3-related protein|FRAP-related protein 1|ATR|FRP1
Gene ID
545
UniProt
Q13535
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Ataxia telangiectasia and Rad3 related
Target
ATR
Clonality
Polyclonal
Isotype
IgG
Applications
ELISA, WB, IHC, IF
Field of Research
Epigenetics, Metabolism, Developmental biology
Purification
Immunogen affinity purified
Dilution
WB: 1:300-1:1000; IP: 1:200-1:1000; IHC: 1:50-1:500; IF: 1:50-1:500
Purity
≥95% as determined by SDS-PAGE
Form
Liquid
Molecular Weight
250-290 kDa
Shipping Conditions
4°C with ice bag
Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)
Tested Applications
ELISA, WB, IHC, IF
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