NEFH Antibody

Product Specifications

Product Name Alternative

NEFH, 200 kDa neurofilament protein, KIAA0845, NFH, NF-H

UniProt

P12036

Reactivity

Gallus, Human, Mouse, Porcine, Rat

Immunogen

The triton-X 100 insoluble protein fraction of rat brain was used as the immunogen for this Neurofilament Heavy antibody.

Target

NEFH

Clonality

Monoclonal

Clone

RT97

Conjugation

Unconjugated

Field of Research

Neuroscience

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Flow Cytometry: 0.5-1 ug/million cellsIHC (FFPE) : 0.5-1 μg/mL for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.