NLRP3 Antibody
NLRP3 Antibody
Product Specifications
Product Name Alternative
NLRP3, NLR family, pyrin domain containing 3, AGTAVPRL, AII, AII/AVP, AVP, C1orf7, CIAS1, CLR1.1, FCAS, FCU, FLJ95925, MWS, NALP3, PYPAF1, AII/AVP receptor-like, NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3, NACHT, LRR and PYD contain
UniProt
Q96P20
Reactivity
Human
Immunogen
The immunogen for this antibody is: RDLYEKAKRDEPK-C
Target
NLRP3
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 36kDa band in Human Peripheral Blood Mononucleocytes lysates after 1μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the smallest calculated size of 106kDa according to NP_899632.1. The 36kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human peripheral blood leukocytes. Recommended concentration: 5μg/mL.
Tested Applications
ELISA, IHC
NCBI Accession Number
NP_001120934.1, NP_001120933.1, NP_004886.3, NP_899632.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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