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C14orf68 Antibody

C14orf68 Antibody

Product Specifications

Product Name Alternative

C14orf68, chromosome 14 open reading frame 68 , HDMCP, HMFN1655 , hepatocellular carcinoma-downregulated mitochondrial carrier protein

UniProt

Q6Q0C1

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-RYGNPDAKPTKAD

Target

C14orf68

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 45kDa band in Human Liver lysates after 0.3μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 33.4kDa according to NP_997000.2. The 45kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Liver shows staining of nucleoli in select hepatocytes. Recommended concentration, 2.5μg/mL.

Tested Applications

ELISA, IHC

NCBI Accession Number

NP_997000.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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