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GOT1 Antibody

GOT1 Antibody

Product Specifications

Product Name Alternative

GOT1, glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1), GIG18, aspartate aminotransferase 1, growth-inhibiting protein 18

UniProt

P17174

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-RSYRYWDAEKR

Target

GOT1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 40kDa band observed in Human Brain (Cerebellum), Liver and Skeletal Muscle lysates (calculated MW of 46.2kDa according to NP_002070.1) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Berk et al, Proc Natl Acad Sci U S A. 1990 May;87 (9) :3484-8; PMID: 2185471) . Recommended concentration: 0.01-1μg/mL.Immunohistochemistry:In paraffin embedded Human Heart shows strong staining of the capillary network between the myocardial fibres in transverse secion. Recommended concentration: 2-3μg/mL. Paraffin embedded Human Kidney. Recommended concentration: 5μg/mL.

Prediction Reactivity

Rat

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

NP_002070.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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