HTATSF1 Antibody
HTATSF1 Antibody
Product Specifications
Product Name Alternative
HTATSF1, HIV-1 Tat specific factor 1 , RP1-196E23.2, TAT-SF1, dJ196E23.2, HIV TAT specific factor 1, cofactor required for Tat activation of HIV-1 transcription
UniProt
O43719
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-QELYGDGKDGDTQTD
Target
HTATSF1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Epigenetics & Chromatin
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 140kDa band observed in lysates of cell line Daudi (calculated MW of 85.9kDa according to NP_055315.2) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Zhou and Sharp, Science. 1996 Oct 25;274 (5287) :605-10.; PMID: 8849451) . Recommended concentration: 1-3μg/mL. An additional band of unknown identity was also consistently observed at 20kDa. This band was successfully blocked by incubation with the immunising peptide.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Prediction Reactivity
Canine, Rat
Tested Applications
ELISA, WB
NCBI Accession Number
NP_055315.2
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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