IFT88 Antibody

IFT88, polaris, TTC10, tetratricopeptide repeat domain 10, HGNC:20606, D13S1056E, DAF19, IFT88, MGC26259, RP11-172H24.2, TG737, hTg737, TPR repeat protein 10, Probe hTg737 (polycystic kidney disease, autosomal recessive), Tg737 protein, intraflagellar transport 88 homolog, recessive polycystic kidney disease protein Tg737 homolog, intraflagellar transport 88 homolog (Chlamydomonas)

Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 85-90kDa band observed in lysates of cell lines HepG2 and NIH3T3 and in Human Kidney lysates (calculated MW of 85.3kDa according to Human NP_001340507.1 and 90.4kDa according to NP_001340498.1, and 93.1kDa according to Mouse NP_033402.2 93. and 89.2kDa according to XP_006518873.1) . Recommended concentration: 0.3-1μg/mL. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Kidney shows staining of the brush border in PCT. Recommended concentration: 3μg/mL.Immunofluorescence: Expression of the protein seen within the nucleus and cytoplasm of HepG2 cells. Recommended concentration: 10μg/mL. This antibody has been successfully used in IF on Mouse Olfactory and Respiratory Epithelium cells as described in the following paper: Miyoshi et al, FASEB J. 2009 Oct;23 (10) :3289-97, PMID: 19470799. Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10μg/mL.

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.