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LRP6 Antibody

LRP6 Antibody

Product Specifications

Product Name Alternative

LRP6, low density lipoprotein receptor-related protein 6, low density lipoprotein-related protein 6, ADCAD2, FLJ90062, FLJ90421

UniProt

O75581

Reactivity

Human

Immunogen

The immunogen for this antibody is: CMTSVATAKGYTSDL

Target

LRP6

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments gave bands at approx 50kDa and 35kDa in Human Adipose lysates after 0.1μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 180kDa according to NP_002327.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) .Immunohistochemistry:In paraffin embedded Human Kidney shows luminar membrane staining in DCT. Recommended concentration: 4-6μg/mL.

Prediction Reactivity

Canine, Mouse, Rat

Tested Applications

ELISA, IHC

NCBI Accession Number

NP_002327.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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