PDE5A Antibody
PDE5A Antibody
Product Specifications
Product Name Alternative
PDE5A, CN5A, PDE5, PDE5A1, CGB-PDE, phosphodiesterase 5A, cGMP-specific, cGMP-binding cGMP-specific 3',5'-cyclic nucleotide phosphodiesterase
UniProt
O76074
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-LINGESGQAKRN
Target
PDE5A
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Signal Transduction
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:In transfected HEK293 transiently expressing PDE5A isoform 2 a band of approx. 95kDa is observed. This band is not observed in the non-transfected HEK293 (calculated MW of 94.8kDa according to isoform 2, NP_236914.2) . Recommended concentration: 0.3-1μg/mL.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/mL. Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10μg/mL.
Prediction Reactivity
Bovine, Canine, Rat
Tested Applications
ELISA, FC, IF, WB
NCBI Accession Number
NP_001074.2, NP_237223, NP_246273.2, NP_236914.2
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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