BAG4 Antibody
BAG4 Antibody
Product Specifications
Product Name Alternative
BAG4, BCL2-associated athanogene 4, BAG-4, SODD, BAG-family molecular chaperone regulator-4, silencer of death domains
UniProt
O95429
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-GNATSDHPNNQDQSS
Target
BAG4
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology, Signal Transduction
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:8000. Western Blot: Approx. 60kDa band observed in cell lines HEK293 and HeLa lysates (calculated MW of 49.6kDa according to NP_004865.1) . The observed molecular weight corresponds to earlier findings in literature with different antibodies. Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 20kDa. This band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry: Paraffin embedded Human Placenta. Recommended concentration: 3.75ug/ml.
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_004865.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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