Recombinant mouse BACE-2 protein

BACE-2, also known as beta secretase 2, belongs to the peptidase A1 family. It is an aspartic protease that is a close homolog of BACE-1. However, BACE-2 differs from BACE-1 in several aspects including pro-enzyme activation, substrate binding sites, transcriptional regulation, and function in Aβ peptide production. It has been shown to play a key role in insulin receptor trafficking in the pancreas where it is expressed in β cells and affects glucose tolerance and was suggested as a promising target for improving β cell function in diabetes. Recombinant mouse BACE-2, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 20 pmol/min/ug in which one unit will convert 1.0pmole of Mca-SEVNLDAEFRK (Dnp) RR-NH2 to Mca- Pro-Leu-OH per minute at pH 3.5 at 25C.

Product Name Alternative

ARP1, ASP1, ASP21, BAE2, CDA13, CEAP1, DRAP, 1110059C24Rik, AEPLC, AI850424, ALP56, beta-site APP-cleaving enzyme 2, beta-secretase 2, Aspartyl protease 1, Asp 1, Beta-site amyloid precursor protein cleaving enzyme 2, Memapsin-1, Membrane-associated aspartic protease 1, Theta-secretase

Expression System

HEK293

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.25 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 20 pmol/min/ug in which one unit will convert 1.0pmole of Mca-SEVNLDAEFRK (Dnp) RR-NH2 to Mca- Pro-Leu-OH per minute at pH 3.5 at 25C.

Molecular Weight

48.6kDa (449aa)

Additionnal Information

ATGP4110-10 µg, ATGP4110-20 µg, ATGP4110-50 µg, ATGP4110-100 µg, ATGP4110-250 µg, ATGP4110-500 µg, ATGP4110-1 mg, ATGP4110-10, ATGP4110-20, ATGP4110-50, ATGP4110-100, ATGP4110-250, ATGP4110-500, ATGP4110-1