HER2 (Phospho-Tyr1221/Tyr1222) Colorimetric Cell-Based ELISA Kit

The HER2 (Phospho-Tyr1221/Tyr1222) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor HER2 (Phospho-Tyr1221/Tyr1222) protein expression profile in cells. The kit can be used for measuring the relative amounts of HER2 (Phospho-Tyr1221/Tyr1222) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on HER2 (Phospho-Tyr1221/Tyr1222) .

Product Specifications

Synonyms

Receptor tyrosine-protein kinase erbB-2;2.7.10.1; Metastatic lymph node gene 19 protein; MLN 19; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; CD340; ERBB2; HER2, MLN19, NEU, NGL

Gene Name

ERBB2

UniProt

P04626

Reactivity

Human, Mouse, Rat

Tissue Specificity

Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.

Applications

ELISA

Detection Range

> 5000 cells/well

Function

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs) . Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.

Molecular Weight

137910 MW

Shipping Conditions

Available

Storage Conditions

Store at 4°C for up to 6 months.

Product Datasheet

https://www.bosterbio.com/datasheet?sku=EKC2426

Product MSDS

https://www.bosterbio.com/msds?sku=EKC2426

Other Gene Names

Receptor tyrosine-protein kinase erbB-2

Subcellular Location

Isoform 1: Cell membrane; Single-pass type I membrane protein. Cytoplasm, perinuclear region. Nucleus. Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1.