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UBE2O Antibody

UBE2O Antibody

Product Specifications

Product Name Alternative

UBE2O, ubiquitin-conjugating enzyme E2O, E2-230K, FLJ12878, KIAA1734

UniProt

Q9C0C9

Reactivity

Human

Immunogen

The immunogen for this antibody is: ALLEAGMPECTEDK

Target

UBE2O

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments in Human Colon lysates and in lysastes of cell line HeLa gave no specific signal but low background (at antibody concentration up to 1μg/mL) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunohistochemistry:In paraffin embedded Human Heart shows textured staining in some myocardial fibres. Recommended concentration, 4-6μg/mL.

Prediction Reactivity

Bovine, Canine, Mouse, Porcine, Rat

Tested Applications

ELISA, IHC

NCBI Accession Number

NP_071349.3

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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