Anti-ASK1 Antibody

Rabbit Polyclonal Antibody specific to ASK1 (CT)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

EC 2.7.11.25, Apoptosis signal-regulating kinase 1, ASK-1, MAPK/ERK kinase kinase 5, MEK kinase 5, MEKK 5

Gene Name

MAP3K5

Gene ID

4217

NCBI Gene ID

UniProt

Q99683

Accession Number

NP_005914

Cellular Locus

Cytoplasm, Endoplasmic reticulum. Note=Interaction with 14-3-3 proteins alters the distribution of MAP3K5/ASK1 and restricts it to the perinuclear endoplasmic reticulum region.

Host

Rabbit

Reactivity

Human

Immunogen

Peptide corresponding to aa 1356-1375 of human ASK1. This peptide sequence is different from that of mouse in the last two amino acids.

Target Antigen

Mitogen-activated protein kinase kinase kinase 5

Target

ASK1

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, ICC, IF

Field of Research

Apoptosis

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. Mediates signal transduction of various stressors like oxidative stress as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) or lipopolysaccharide (LPS). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs). Both p38 MAPK and JNKs control the transcription factors activator protein-1 (AP-1). {PubMed:10411906, PubMed:10688666, PubMed:10849426, PubMed:11029458, PubMed:11154276, PubMed:11689443, PubMed:11920685, PubMed:14688258, PubMed:14749717, PubMed:15023544, PubMed:16129676, PubMed:17220297, PubMed:23102700, PubMed:26095851, PubMed:8940179, PubMed:8974401, PubMed:9564042, PubMed:9774977}.

Additionnal Information

Immunoblotting: use at 0.5-1ug/mL Immunocytochemistry: use at 10ug/mL. These are recommended concentrations. Enduser should determine optimal concentration for their application. Positive control: Whole cell lysate from SW1353 cells.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes human ASK1 (155kDa).

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (apoptosis signal-regulating kinase 1) and MAPKKK5. ASK1 activates two different subgroups of MAPKK, MKK4 and MKK6, which in turn activate c-jun N-terminal kinase (JNK) and p36 MAP kinase. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induces apoptotic cell death. A catalytically inactive form of ASK1 inhibits TNF -induced apoptosis. ASK1 is expressed in a variety of human and mouse tissues.