CINK4

CINK4 is a triaminopyrimidine derivative that inhibits Cdk4/cyclin D1 and Cdk6/cyclin D1 activity with IC₅₀ values of 1.5 and 5.6 μM, respectively. It reduces retinoblastoma protein phosphorylation at Ser780 and Ser795 and induces cell cycle arrest in the G1 phase in U2OS and MRC-5 cells at 5-10 µM. Administration of CINK4 at 30 mg/kg, i.p. for 29 days, suppresses tumor growth in mice bearing human HCT116 colon carcinoma xenografts.