Anti-Thymidylate synthase Antibody

Mouse Monoclonal Antibody specific to Thymidylate Synthase

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

TS, TSase, EC 2.1.1.45

Gene Name

TYMS

NCBI Gene ID

UniProt

P04818

Cellular Locus

Nucleus, Cytoplasm, Mitochondrion, Mitochondrion matrix, Mitochondrion inner membrane

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human thymidylate synthase produced in E. coli.

Target Antigen

Thymidylate synthase

Target

Thymidylate synthase

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IP, IHC, ELISA

Field of Research

Cancer research

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Catalyzes the reductive methylation of 2'-deoxyuridine 5'-monophosphate (dUMP) to thymidine 5'-monophosphate (dTMP), using the cosubstrate, 5,10- methylenetetrahydrofolate (CH2H4folate) as a 1-carbon donor and reductant and contributes to the de novo mitochondrial thymidylate biosynthesis pathway. {PubMed:11278511, PubMed:21876188}.

Additionnal Information

Immunohistochemistry: use at 5-10 ug/ml on frozen sections (reactivity on paraffin-embedded sections has not been determined) Immunoblotting: use at 5-10 ug/ml ELISA: use at 2-5 ug/ml (optimized for TS on solid phase at 5 ug/ml). Immunoprecipitation: use #34002 only at 5-10 ug/ml.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.

Specificity

These antibodies specifically recognize human TS (36 kDa). They do not cross-react with rat TS.

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

Thymidylate synthase (TS) is an intracellular enzyme critical for de novo synthesis of DNA. In cancer, expression of TS is often elevated and becomes further elevated as a result of treatment with the most commonly used chemotherapeutic, 5-fluorouracil (5-FU). Resistance or lack of response to 5-FU is attributed to the elevation of TS activity. Overexpression of TS has been demonstrated in many types of tumors including breast, colon, gastric and melanoma and is usually predictive of shortened survival time.