Urine Exosome and Free-Circulating RNA Isolation Maxi Kit

Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias Versatile sample input ranges Isolate all sizes of free-circulating RNA, including microRNA​ Bind and elute all RNA irrespective of size or GC content, without bias The purified exosomal RNA is free from any circulating RNA-binding proteins No phenol extractions, Proteinase K treatment nor carrier RNA required No time-consuming ultracentrifugation, filtration nor special syringes are required No precipitation reagents nor overnight incubation required Concentrate isolated exosomal RNA and free-circulating RNA into a flexible elution volume ranging from 50 µL to 100 µL Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix These kits provide a fast, reliable and convenient method to sequentially isolate and concentrate exosomal RNA as well as Free-Circulating RNA from different urine sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. These kits are designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. These kits provide a clear advantage over other available kits since they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kits allow the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. Moreover, the free-circulating, protein-bound, RNA is free from any exosomal RNA. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays. Urine Exosome and Free-Circulating RNA Isolation Mini Kit For sample volumes ranging from 250 µL to 1 mL. Urine Exosome and Free-Circulating RNA Isolation Midi Kit For sample volumes ranging from 2 mL to 10 mL. Urine Exosome and Free-Circulating RNA Isolation Maxi Kit For sample volumes ranging from 11 mL to 30 mL.