Mouse ACE/Cd143 ELISA Kit PicoKine®

Angiotensin-converting enzyme (ACE), an exopeptidase, is a circulating enzyme that participates in the body's renin-angiotensin system (RAS), which mediates extracellular volume (i.e. that of the blood plasma, lymph and interstitial fluid), and arterial vasoconstriction. It is secreted by pulmonary and renal endothelial cells and catalyzes the conversion of decapeptide angiotensin I to octapeptide angiotensin II. Using a DNA marker at the growth hormone gene locus, which they characterized as 'extremely polymorphic' and which showed no recombination with ACE, ACE was mapped to 17q22-q24, consistent with the in situ hybridization mapping to 17q23. ACE, or kininase II, is a dipeptidyl carboxypeptidase that plays an important role in blood pressure regulation and electrolyte balance by hydrolyzing angiotensin I into angiotensin II, a potent vasopressor, and aldosterone-stimulating peptide. The enzyme is also able to inactivate bradykinin, a potent vasodilator.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 157 139 140 158 148 9.01 6% Sample 2 733 727 809 711 745 37.81 5% Sample 3 3455 3416 3214 3153 3309 128.57 3.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 157 139 140 158 148 9.01 6% Sample 1 157 139 140 158 148 9.01 6% Sample 2 733 727 809 711 745 37.81 5% Sample 2 733 727 809 711 745 37.81 5% Sample 3 3455 3416 3214 3153 3309 128.57 3.8% Sample 3 3455 3416 3214 3153 3309 128.57 3.8%

Angiotensin-converting enzyme; ACE;3.2.1.-;3.4.15.1; Dipeptidyl carboxypeptidase I; Kininase II; CD143; Angiotensin-converting enzyme, soluble form; Ace; Dcp1

Cancer, Cancer Metabolism, Cardiovascular, Cell Biology, Cofactors, Vitamins/Minerals, Hematopoietic Progenitors, Metabolism, Metabolism Processes, Metalloprotease, Pathways and Processes, Proteolysis/Ubiquitin, Proteolytic Enzymes, Response To Hypoxia, Serum Proteins, Signal Transduction, Stem Cells, Surface Molecules, Vasculature, Vitamins/Minerals

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 157 733 3455 155 795 3149 Standard deviation 6.9 40.31 272.94 7.59 46.11 286.55 CV (%) 4.4% 5.5% 7.9% 4.9% 5.8% 9.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 157 733 3455 155 795 3149 Standard deviation 6.9 40.31 272.94 7.59 46.11 286.55 CV (%) 4.4% 5.5% 7.9% 4.9% 5.8% 9.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 157 733 3455 155 795 3149 Mean (pg/ml) 157 733 3455 155 795 3149 Standard deviation 6.9 40.31 272.94 7.59 46.11 286.55 Standard deviation 6.9 40.31 272.94 7.59 46.11 286.55 CV (%) 4.4% 5.5% 7.9% 4.9% 5.8% 9.1% CV (%) 4.4% 5.5% 7.9% 4.9% 5.8% 9.1%

Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. This GPIase activity seems to be crucial for the egg-binding ability of the sperm. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.026 0.058 0.098 0.169 0.32 0.605 1.211 1.812 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.026 0.058 0.098 0.169 0.32 0.605 1.211 1.812 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.026 0.058 0.098 0.169 0.32 0.605 1.211 1.812 O.D. 0.026 0.058 0.098 0.169 0.32 0.605 1.211 1.812

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.