SIRT3 Antibody
Zinc-fingers and homeobox (ZHX) proteins are transcription factors that interact with the activation domain of the A subunit of nuclear factor-Y (NF-YA) . ZHX1-3 are ubiquitously expressed proteins expressed in various tissues. They act as transcriptional repressors and localize to the nucleus. The ZHX proteins contain two Cys2-His2-type zinc-finger motifs and five homeodomains (HDs) . These domains allow the ZHX proteins to form homodimers, but they can also form heterodimers with each other. However, this dimerization is not required for repressor activity. Hypermethylation-mediated silencing of ZHX2 is an epigenetic event involved in hepatocellular carcinoma (HCC) .
Product Specifications
CAS Number
9007-83-4
Specifications
ELISA (order BSA-free format for coating), Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL
UniProt
Q9NTG7
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full-length human SIRT3 protein was used as the immunogen for the SIRT3 antibody.
Clonality
Monoclonal
Isotype
IgG2a
Clone
PCRP-SIRT3-1C10
Applications
ELISA, FACS, IF, WB
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the SIRT3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This SIRT3 antibody is available for research use only.
Storage Conditions
Aliquot the SIRT3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the SIRT3 antibody should be determined by the researcher.
Location
Cytoplasm
Image Legend
Immunofluorescent staining of PFA-fixed human HeLa cells using SIRT3 antibody (green, clone PCRP-SIRT3-1C10) and phalloidin (red) .
Frequently Asked Questions
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