Tyrosinase Antibody
Recognizes a cluster of proteins between 70-80kDa, identified as tyrosinase. Occasionally a minor band at 55kDa is also detected. This MAb shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway; the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this MAb shows tyrosinase in melanotic as well as amelanotic variants.This MAb is a useful marker for melanocytes and melanomas.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P14679
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full-length human Tyrosinase protein was used as the immunogen for the Tyrosinase antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
TYR/3826
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Aliquot the Tyrosinase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Tyrosinase antibody is available for research use only.
Storage Conditions
Aliquot the Tyrosinase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the Tyrosinase antibody should be determined by the researcher.
Location
Cytoplasm
Image Legend
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
CD4 (T-Helper/Inducer Cell Marker) (CD4/3026), CF640R conjugate, 0.1mg/mL
