Growth Differentiation Factor 9 Antibody / GDF9

GDF9 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Growth factors synthesized by ovarian somatic cells directly affect oocyte growth and function. GDF9 is expressed in oocytes and is thought to be required for ovarian folliculogenesis. GDF9/6458 can be used in assays to detect oocyte expression and has been shown to neutralize GDF9 biological activity.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

O60383

Host

Mouse

Reactivity

Human

Immunogen

A recombinant fragment (within amino acids 250-450) of human Growth Differentiation Factor 9 protein was used as the immunogen for the Growth Differentiation Factor 9 antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

GDF9/6458

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Growth Differentiation Factor 9 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Growth Differentiation Factor 9 antibody is available for research use only.

Storage Conditions

Aliquot the Growth Differentiation Factor 9 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Growth Differentiation Factor 9 antibody should be determined by the researcher.

Location

Cytoplasm, Secreted

Image Legend

IHC staining of FFPE human ovary tissue with Growth Differentiation Factor 9 antibody (clone GDF9/6458) . Inset: PBS used in place of primary Ab (secondary Ab negative control) .