MART-1 Antibody / Melan-A

MLANA (Melan-A, also known as MART1; MART-1), located on 9p24.1, is a Protein Coding gene. The gene produces a 13157 Da protein composed of 118 amino acids. MLANA is a hydrophobic transmembrane protein without glycosylation sites highly enriched in early melanosomes. MLANA is expressed in skin and retinae melanocytes and the majority of melanoma tumors, but it is absent from other tissues and tumors. Diseases such as Melanoma In Congenital Melanocytic Nevus and Angiomyolipoma are associated with MLANA. The related pathways of MLANA include NF-kappaB Signaling.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

UniProt

Q16655

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

An E.coli-derived human recombinant protein (M1-L37) was used as the immunogen for the MART-1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the MART-1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This MART-1 antibody is available for research use only.

Storage Conditions

After reconstitution, the MART-1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the MART-1 antibody should be determined by the researcher.

Image Legend

Flow cytometry testing of fixed and permeabilized human HEL cells with MART-1 antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= MART-1 antibody.