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NFE2 Antibody / Nuclear factor erythroid-derived 2 45 kDa subunit

Transcription factor NF-E2 45 kDa subunit is a protein that in humans is encoded by the NFE2 gene. Enables several functions, including WW domain binding activity; identical protein binding activity; and protein N-terminus binding activity. Contributes to cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to act upstream of or within several processes, including labyrinthine layer blood vessel development; negative regulation of bone mineralization; and negative regulation of syncytium formation by plasma membrane fusion. Part of protein-DNA complex.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q16621

Host

Rabbit

Reactivity

Human

Immunogen

E. coli-derived recombinant human protein (amino acids E20-D373) was used as the immunogen for the NFE2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the NFE2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This NFE2 antibody is available for research use only.

Storage Conditions

After reconstitution, the NFE2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the NFE2 antibody should be determined by the researcher.

Image Legend

Western blot testing of human 1) K562 and 2) HepG2 cell lysate with NFE2 antibody. Predicted molecular weight ~41 kDa, observed at 41-45 kDa.

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