TAPP-1 Antibody / PLEKHA1

Pleckstrin homology domain-containing family A member 1, also called Tandem PH domain-containing protein 1 (TAPP-1), is a protein that in humans is encoded by the PLEKHA1 gene. This gene encodes a pleckstrin homology domain-containing adapter protein. The encoded protein is localized to the plasma membrane where it specifically binds phosphatidylinositol 3,4-bisphosphate. This protein may be involved in the formation of signaling complexes in the plasma membrane. Polymorphisms in this gene are associated with age-related macular degeneration. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 5.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q9HB21

Host

Rabbit

Reactivity

Human

Immunogen

E. coli-derived recombinant human protein (amino acids K76-K387) was used as the immunogen for the TAPP-1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the TAPP-1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This TAPP-1 antibody is available for research use only.

Storage Conditions

After reconstitution, the TAPP-1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the TAPP-1 antibody should be determined by the researcher.

Location

Cytoplasmic, nuclear, cell membrane

Image Legend

Immunofluorescent staining of FFPE human lung cancer tissue with TAPP-1 antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH8 EDTA buffer for 20 min.