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Anti-Mouse CD8 Beta (53-5.8) In Vivo Antibody - Ultra-Low Endotoxin

Product Specifications

Background

CD8b is the 32 kD β chain of CD8, also known as Lyt-3.2 or Ly-3.2. It is a member of the Ig superfamily expressed as a heterodimer with the CD8A chain on a subset of MHC class I-restricted T cells and most thymocytes. CD8 is a co-receptor for the TCR complex involved in T cell activation.

Specifications

Anti-CD8 beta In Vivo Antibody - Ultra Low Endotoxin (53-5.8) recognizes an epitope on Mouse CD8b.2

Product Name Alternative

Cd8b, Lymphocyte antigen 3, T-cell membrane glycoprotein Ly-3, T-cell surface glycoprotein Lyt-3, Cd8b1, Ly-3, Lyt-3, Lyt3

UniProt

P10300

Host

Rat

Species Reactivity

Mouse

Target

CD8 beta

Isotype

IgG1

Clone

53-5.8

Applications

Western Blot, Functional Assays, Flow Cytometry, Immunocytochemistry, IHC (Frozen), Immunoprecipitation

Purification Method

This monoclonal antibody was purified using Protein G

Concentration

1.0 - 5.0 mg/mL

Endotoxin

≤ 0.5 EU/mg as determined by the LAL method

Purity

>98% by SDS-PAGE and HPLC

Buffer

ICH3001-100mL ICH3002-100mL ICH3003-100mL

Shipping Conditions

Blue ice

Storage Conditions

Anti-CD8 beta In Vivo Antibody - Ultra Low Endotoxin (53-5.8) is stable when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 80°C. Avoid Repeated Freeze Thaw Cycles.

Product Datasheet

https://ichor.bio/productpdf/print/index/url_key/Anti-CD8-beta-In-Vivo-Antibody-Low-Endotoxin-53-5.8-ICH1046/

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.4 and does not contain BSA or sodium azide as a preservative.

Antigen Distribution

The antibody 53-5.8 is specific for Ly-3.2 and has low reactivity with Ly-3.1 and is found on the majority of thymocytes, cytotoxic T cell subsets

Aggregation

Aggregation level ≤ 1%

Applications Notes

Each investigator should determine their own optimal working dilution for specific applications.

Isotype Control

https://www.ichor.bio/product/Rat-IgG1-Isotype-Control-for-In-Vivo-Low-Endotoxin-GL113-ICH2246

CAS Number

9007-83-4

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