Phospho-MYPT1 Antibody (Ser668)

Key regulator of protein phosphatase 1C (PPP1C) . Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN- dependent suppression of HIF1A activity. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000

UniProt

O14974

Host

Rabbit

Reactivity

Human

Immunogen

Amino acids surrounding phosphorylated serine 668 from human MYPT1 were used as the immunogen for the phospho-MYPT1 antibody.

Clonality

Polyclonal

Isotype

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the phospho-MYPT1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This phospho-MYPT1 antibody is available for research use only.

Storage Conditions

Aliquot the phospho-MYPT1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the phospho-MYPT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Chicken, Mouse, Rat

Image Legend

Western blot analysis of lysates from human HeLa and Jurkat cell lines, untreated or treated with Calyculin A (100nM), using phospho-MYPT1 antibody (upper) or Tubulin Ab (lower) .