B5R Recombinant Protein

Product Specifications

Background

Monkeypox is a rare zoonosis caused by monkeypox virus, which has become the most serious orthpoxvirus and consists of complex double stranded DNA. The cases are mostly in central and western Africa. The pathogenesis of monkeypox is that the virus invades the body from respiratory mucosa , multiplies in lymphocytes, and incurs into blood producing transient venereal toxemia. after the virus multiplies in cells, the cells can invade the blood and propagate to the skin of the whole body, causing lesions.

CAS Number

9000-83-3

Swiss Prot

Q3I8J3

Modification Site

NdeI-XhoI

Expression System

Pet-22b (+)

Tag

His-tag; Sumo-Tag

Purity

Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE) .

Solubility

0.5M Urea, PH7.4

Molecular Weight

~53kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Host or Source

E.coli

AA Sequence

HMDFFKKEILDWSIYLFLHYITRLCSNSSNSSTSHIIQEYNLVRKYEKVDKTIVDFLSRWPNLFHILEYGENILHIYFIDAANTNIMIFFLDRVLNINKNRGSFIHNLGLSSINIKEYVYQLVNNDHLDNSIRLMLENGRRTRHFLSYILDTVNIYISILINHRFYIDAEDSYGCTLLHRCIYNYRKSESESYNELIKILLNNGSDVDKKDTYGNTPFILLCKHDIDNAELFEICLENANIDSVDFNGYTPLHYVSCRNKYDFVKLLISKGANVNARNRFGTTPFYCGIIHGISLIKLYLESDTELEIDNEHIVRHLIIFDAVESLDYLLSRGVIDINYRTIYNETSIYDAVSYNAYNTLVYLLNRNGDFETITTSGCTCISEAVANNNKIIMDILLSKRPSLKIMIPSMIAITKHKQHNADLLKMCIKYTACMTDYDTLIDVQSLHQYKWYILKCFDEIDIMKRCYIKNKTVFQLVFCIKDINTLMRYGRHPSFVKCNILDVYGSHVRNIIASIRYRQRLISLLSKKLDAGDKWSCFPNEIKYKILENFNDNELTTYLKILLE