UBE3B polyclonal antibody
Product Specifications
Background
Ubiquitin-protein ligase E3B, E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Ubiquitin-protein ligase E3B; HECT-type ubiquitin transferase E3B; UBE3B
Swiss Prot
Q7Z3V4
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding Human UBE3B.
Conjugation
Unconjugated
Related Pathways
Ubiquitin Proteasome
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 123 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
UBE3B polyclonal antibody detects endogenous levels of UBE3B protein.
Applications Notes
Western blot (WB) analysis of UBE3B polyclonal antibody at 1:500 dilution Lane1:PC12 whole cell lysate (40ug) Lane2:CT26 whole cell lysate (40ug) Lane3:SGC7901 whole cell lysate (40ug) Lane4:MCF-7 whole cell lysate (40ug) Lane5:HEK293T whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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