CAGE1 polyclonal antibody
Product Specifications
Background
Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop. LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin-2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and stabilizing the actin cytoskeleton. In this way LIMK1 regulates several actin-dependent biological processes including cell motility, cell cycle progression, and differentiation. Phosphorylates TPPP on serine residues, thereby promoting microtubule disassembly. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. Required for atypical chemokine receptor ACKR2-induced phosphorylation of cofilin (CFL1) .
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Cancer-associated gene 1 protein; CAGE-1; Cancer/testis antigen 3; CT3
Swiss Prot
Q8TC20
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 718-762 of Human CAGE1.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 96% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 90 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
CAGE1 polyclonal antibody detects endogenous levels of CAGE1 protein.
Applications Notes
Western blot (WB) analysis of CAGE1 polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:The testis tissue lysate of Mouse (40ug) Lane4:The testis tissue lysate of Rat (40ug) Lane5:H9C2 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
Curated Selection
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