TBK1 polyclonal antibody
Product Specifications
Background
The transcription factor NFκB is retained in the cytoplasm in an inactive form by the inhibitory protein IκB. Activation of NFκB requires that IκB be phosphorylated on specific serine residues, which results in targeted degradation of IκB. IκB kinase α (IKKα), previously designated CHUK, interacts with IkB-a and specifically phosphorylates IκB-α on the sites that trigger its degradation, serines 32 and 36. The functional IKK complex contains three subunits, IKKα, IKKβ and IKKγ (also designated NEMO), and each appear to make essential contributions to IκB phosphorylation. TANK binding kinase (TBK1), also designated T2K, is a novel IKK-related kinase that has been identified in murine and human tissues. TBK1 was shown to complex with TRAF2 and TANK in the NFκB activation pathway. TBK1 shares homology with IKKα and IKKβ in the amino-terminal half, which includes the kinase domain.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Serine/threonine-protein kinase TBK1; NF-kappa-B-activating kinase; T2K; TANK-binding kinase 1; TBK1; NAK
Swiss Prot
Q9UHD2
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to residues in Human TBK1
Conjugation
Unconjugated
Related Pathways
NF-kB Signaling, Immune Cell Signaling, STING Signaling, Toll-like Receptor signaling
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 84 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
TBK1 polyclonal antibody detects endogenous levels of TBK1 protein.
Applications Notes
Western blot (WB) analysis of TBK1 polyclonal antibody at 1:500 dilution Lane1:SK-OVCAR3 whole cell lysate (40ug) Lane2:HCT116 whole cell lysate (40ug) Lane3:CT26 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
Curated Selection
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