AICDA polyclonal antibody
Product Specifications
Background
Activation-induced Cytidine Deaminase (AID, HIGM-2) is a 198-amino acid, RNA-editing enzyme that contains a conserved cytidine deaminase motif and plays an important role in B-cell terminal differentiation. AID is expressed in germinal center B cells and contributes to the production of neutralizing antibodies IgG, IgA, and IgE. Hyper-IgM syndrome (HIGM2) patients that have deficient levels of AID show the absence of immuno-globulin class switch recombination (CSR), lack of immuno-globulin somatic hypermutations, and lymph node hyperplasia mediated by the presence of giant germinal centers. Furthermore, AID-/- mice are defective in CSR and also show a hyper-IgM phenotype, characterized by enlarged germinal centers containing active B cells. AID thus appears to be required in several stages of B-cell terminal differentiation that are necessary for efficient antibody responses such as B cell proliferation, immunoglobulin somatic hypermutations and CSR.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Single-stranded DNA cytosine deaminase; Activation-induced cytidine deaminase; Cytidine aminohydrolase; AICDA; AID
Swiss Prot
Q9GZX7
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to residues in Human AICDA
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 24 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
AICDA polyclonal antibody detects endogenous levels of AICDA protein.
Applications Notes
Western blot (WB) analysis of AICDA polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate Lane2:sp2/0 whole cell lysate Lane3:PC12 whole cell lysate
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
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