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ORC1 polyclonal antibody

Product Specifications

Background

ORC proteins bind specifically to origins of replication where they serve as scaffold for the assembly of additional initiation factors. Human ORC subunits 1-6 are expressed in the nucleus of proliferating cells and tissues, such as the testis. ORC1 and ORC2 are both expressed at equivalent concentrations throughout the cell cycle; however, only ORC2 remains stably bound to chromatin. ORC4 and ORC6 are also expressed constantly throughout the cell cycle. ORC2, ORC3, ORC4 and ORC5 form a core complex upon which ORC6 and ORC1 assemble. The formation of this core complex suggests that ORC proteins play a crucial role in the G1-S transition in mammalian cells.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Origin recognition complex subunit 1; Replication control protein 1; ORC1L; PARC1

Swiss Prot

Q13415

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 364-409 of Human ORC1.

Conjugation

Unconjugated

Applications

WB

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 97, 120 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

ORC1 polyclonal antibody detects endogenous levels of ORC1 protein.

Applications Notes

Western blot (WB) analysis of ORC1 polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:MCF-7 cell lysate Lane3:PC12 cell lysate

Host or Source

Rabbit

Available Sizes

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