ORC1 polyclonal antibody
Product Specifications
Background
ORC proteins bind specifically to origins of replication where they serve as scaffold for the assembly of additional initiation factors. Human ORC subunits 1-6 are expressed in the nucleus of proliferating cells and tissues, such as the testis. ORC1 and ORC2 are both expressed at equivalent concentrations throughout the cell cycle; however, only ORC2 remains stably bound to chromatin. ORC4 and ORC6 are also expressed constantly throughout the cell cycle. ORC2, ORC3, ORC4 and ORC5 form a core complex upon which ORC6 and ORC1 assemble. The formation of this core complex suggests that ORC proteins play a crucial role in the G1-S transition in mammalian cells.
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Origin recognition complex subunit 1; Replication control protein 1; ORC1L; PARC1
Swiss Prot
Q13415
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 364-409 of Human ORC1.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 97, 120 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
ORC1 polyclonal antibody detects endogenous levels of ORC1 protein.
Applications Notes
Western blot (WB) analysis of ORC1 polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:MCF-7 cell lysate Lane3:PC12 cell lysate
Host or Source
Rabbit
CAS Number
9007-83-4
Available Sizes
Curated Selection
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