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PAK2 (phospho-S20) polyclonal antibody

Product Specifications

Background

Three isoforms of serine/threonine kinases, designated αPAK p68, βPAK p65 and γPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The α, β and γPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. There are eight sites of autophosphorylation on γPAK, including Ser 19, Ser 141 and Thr 402, and phosphorylation of Ser 141 and Thr 402 is correlated with γPAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Serine/threonine-protein kinase PAK 2; Gamma-PAK; PAK65; S6/H4 kinase; p21-activated kinase 2; PAK-2; p58; PAK-2p27; PAK-2p34; C-t-PAK2; PAK2; PAKγ

Swiss Prot

Q13177

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human PAK2 around the phosphorylation site of Serine 20.

Conjugation

Unconjugated

Related Pathways

Regulation of Microtubule Dynamics, Epigenetic Writers and Erasers of Histones H2A H2B and H4

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 60 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-PAK2 (S20) polyclonal antibody detects endogenous levels of PAK2 protein only when phosphorylated at Ser20.

Applications Notes

Western blot (WB) analysis of p-PAK2 (S20) polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate treated with EGF (0.1ng/ML,30mins) Lane2:Raw264.7 cell lysate treated with EGF (0.1ng/ML,30mins) Lane3:H9C2 cell lysate treated with EGF (0.1ng/ML,30mins)

Host or Source

Rabbit

Available Sizes

Curated Selection

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