Cdk1/Cdc2 (phospho-T14) polyclonal antibody
Product Specifications
Background
Cdc2, an evolutionarily conserved serine/threonine-specific protein kinase, is essential in the cell cycle transition from G2 to M phase. Cdc2 is regulated by association with B-type cyclins and by reversible phosophorylation. Cyclin B binding facilitates the phosphorylation of Cdc2 p34 on three regulatory sites: threonine 14, tyrosine 15, and threonine 161. In higher eukaryotes, Cdc2 is negatively regulated by phosphorylation of two residues located in the ATP-binding site, Thr 14 and Tyr 15. Cdc2 is positively regulated by the cyclin-dependent phosphorylation of Thr 161. Both phosphorylation and de- phosphorylation at Thr 161 are required for progression through the cell cycle.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Cyclin-dependent kinase 1; Cell division control protein 2 homolog; Cell division protein kinase 1; p34 protein kinase; CDK1; CDC2; CDC28A; CDKN1; P34CDC2
Swiss Prot
P06493
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human Cdk1/Cdc2 around the phosphorylation site of Threonine 14.
Conjugation
Unconjugated
Related Pathways
Cell Cycle G2 M DNA Damage Signaling Pathway
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 34 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-Cdk1/Cdc2 (T14) polyclonal antibody detects endogenous levels of Cdk1/Cdc2 protein only when phosphorylated at Thr14.
Applications Notes
Western blot (WB) analysis of p-Cdk1/Cdc2 (T14) pAb at 1:500 dilution Lane1:Hela whole cell lysate (40ug) Lane2:PC3 whole cell lysate (40ug) Lane3:The Testis tissue lysate of Mouse (40ug) Lane4:The Testis tissue lysate of Rat (40ug)
Host or Source
Rabbit
Available Sizes
Curated Selection
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