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ZP1 (G259) polyclonal antibody

Product Specifications

Background

The mammalian zona pellucida is composed of three major glycoproteins, ZP1, ZP2 and ZP3. ZP2 has been implicated as a secondary sperm receptor that binds sperm only after the induction of the sperm acrosome reaction. Both ZP2 and ZP3 are modified by the zona reaction; ZP2 undergoes a proteolytic cleavage and ZP3 loses its ability to induce the acrosome reaction and its sperm receptor activity. During the process of fertilization, the initial interaction between male and female gametes is mediated by a sperm receptor, ZP3, which resides in the extracellular glycoprotein matrix (zona pellucida) surrounding the oocyte

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Zona pellucida sperm-binding protein 1; Zona pellucida glycoprotein 1; Zp-1; ZP1

Swiss Prot

P60852

Reactivity

Human

Immunogen

Synthetic peptide, corresponding to amino acids 240-280 of Human ZP1.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 75 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

ZP1 (G259) polyclonal antibody detects endogenous levels of ZP1 protein.

Applications Notes

Western blot (WB) analysis of ZP1 (G259) pAb at 1:1000 dilution Lane1:SK-OVCAR3 whole cell lysate (40ug) Lane2:A2780 whole cell lysate (40ug) Lane3:PC3 whole cell lysate (20ug)

Host or Source

Rabbit

Available Sizes

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