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Recombinant human AST1/GOT1 protein

Glutamic-oxaloacetic transaminase (GOT) is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Recombinant human GOT1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Specifications

Specific activity is > 50 units/mg, and is defined as the amount of enzyme that converts 1umole of a-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25C.

Product Name Alternative

Glutamic-oxaloacetic transaminase 1, Aspartate aminotransferase 1, Aspartate transaminase 1, AST1, SGOT, AST, cAspAT, Cysteine aminotransferase cytoplasmic, Cysteine transaminase, Cytoplasmic, cCAT, Transaminase A

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.5 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 50unit/mg, and is defined as the amount of enzyme that converts 1umole of a-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25C.

Molecular Weight

48.4 kDa (433aa) confirmed by MALDI-TOF

Additionnal Information

GOT1, Glutamic-oxaloacetic transaminase 1, Aspartate aminotransferase 1, Transaminase A, GIG18, ATGP3177-10 µg, ATGP3177-20 µg, ATGP3177-50 µg, ATGP3177-100 µg, ATGP3177-250 µg, ATGP3177-500 µg, ATGP3177-1 mg, ATGP3177-10, ATGP3177-20, ATGP3177-50, ATGP3177-100, ATGP3177-250, ATGP3177-500, ATGP3177-1

References & Citations

Bousquet-Lemercier B., et al. (1990) Biochemistry 29 (22) : 5293-9.; ; Totan A., et al. (2006) Clin Chem Lab Med. 44 (5) :612-5.

Other References

Yoshida T, et al. A covalent small molecule inhibitor of glutamate-oxaloacetate transaminase 1 impairs pancreatic cancer growth. (Biochem Biophys Res Commun. 2020) {https://pubmed.ncbi.nlm.nih.gov/31787239/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 2mM DTT, 100 mM NaCl

Scientific Category

Metabolism

NCBI Accession Number

NP_002070

Uniprot Accession Number

P17174

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MAPPSVFAEV PQAQPVLVFK LTADFREDPD PRKVNLGVGA YRTDDCHPWV LPVVKKVEQK IANDNSLNHE YLPILGLAEF RSCASRLALG DDSPALKEKR VGGVQSLGGT GALRIGADFL ARWYNGTNNK NTPVYVSSPT WENHNAVFSA AGFKDIRSYR YWDAEKRGLD LQGFLNDLEN APEFSIVVLH ACAHNPTGID PTPEQWKQIA SVMKHRFLFP FFDSAYQGFA SGNLERDAWA IRYFVSEGFE FFCAQSFSKN FGLYNERVGN LTVVGKEPES ILQVLSQMEK IVRITWSNPP AQGARIVAST LSNPELFEEW TGNVKTMADR ILTMRSELRA RLEALKTPGT WNHITDQIGM FSFTGLNPKQ VEYLVNEKHI YLLPSGRINV SGLTTKNLDY VATSIHEAVT KIQ
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