MyD88 (V220) polyclonal antibody

Product Specifications

Background

The myeloid differentiation protein MyD88 was originally characterized as a protein upregulated in myeloleukemic cells following IL-6-induced growth arrest and terminal differentiation. MyD88 is now known to function as an adaptor protein for the association of IRAK with the IL-1 receptor. MyD88 is functionally homologous to the adaptor protein tube in the Toll signaling pathway of Drosophilia, and both proteins are members of the Toll/IL-1R superfamily. MyD88 contains a characteristic N-terminal death domain that is essential for NFκB activation and an adjacent Toll/IL-1R homology domain (TIR domain) . Collectively, these domains enable the protein-protein interactions of MyD88 with IRAK and the IL-1 receptor complex.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Myeloid differentiation primary response protein MyD88

Swiss Prot

Q99836

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 186-236 of Human MyD88.

Conjugation

Unconjugated

Related Pathways

Toll-like Receptor signaling

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 35 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

MyD88 (V220) polyclonal antibody detects endogenous levels of MyD88 protein.

Applications Notes

Western blot (WB) analysis of MyD88 (V220) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:PC12 whole cell lysate