Glucosidase IIβ (K117) polyclonal antibody

Trimming of glucoses from N-linked core glycans on newly synthesized glycoproteins occurs sequentially through the action of Glucosidases I and II in the endoplasmic reticulum (ER) . Glucosidase II is an ER-localized enzyme that contains α and β subunits (Glucosidase IIα and Glucosidase IIβ) . The α and β subunits form a defined heterodimeric complex with a molecular weight about 161 kDa. Glucosidase IIα is the catalyitc core of the enzyme and can function independently of the β subunit. The sequence of Glucosidase IIβ encodes protein rich in glutamic and aspartic acid with a putative ER retention signal (HDEL) at the C terminus. The phosphorylated form of Glucosidase IIβ is localized in the plasma membrane and is highly expressed in FGF stimulated fibroblasts and epidermal carcinoma cells. Glucosidase IIβ was first purified from a human carcinoma cell line as a potential substrate for protein kinase C. Through the HDEL signal at the C-terminus, Glucosidase IIβ retains the complete complex in the ER.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of Glucosidase IIβ (K117) pAb at 1:500 dilution Lane1:The Testis tissue lysate of Mouse (40ug) Lane2:The Testis tissue lysate of Rat (40ug) Lane3:SGC7901 whole cell lysate (40ug) Lane4:HCT116 whole cell lysate (40ug) Lane5:A549 whole cell lysate (40ug) Lane6:MCF-7 whole cell lysate (40ug)