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Creatine kinase (CK) Activity Assay Kit

Product Specifications

Target

CK; Creatine phosphokinase

Type

Colorimetric method

Field of Research

Amino Acids and Proteins

Assay Principle

Creatine kinase (CK) catalyze creatine phosphate and ADP to produce creatine and ATP. Hexokinase catalyze creatine and glucose to produce glucose-6-phosphate. Glucose-6-phosphate dehydrogenase (G-6-PD) catalyze glucose-6-phosphate and NADP+ to produce NADPH which have a maximum absorption peak at 340 nm. The CK activity can be calculated by measuring the OD values at 340 nm.

Assay Performance Time

80 min

Sample Type

Serum; plasma; animal tissue; cells

Detection Range

3.7-160 U/L

Sensitivity

3.7 U/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at 2~8°C for 12 months with shading light.

Citation 01

https://www.sciencedirect.com/science/article/pii/S0014299921005069

Citation 02

https://www.sciencedirect.com/science/article/pii/S0026286223000237

Citation 03

https://www.sciencedirect.com/science/article/pii/S0378111920310234

Citation 04

https://www.hindawi.com/journals/jnm/2021/7512957/

Citation 05

https://www.spandidos-publications.com/10.3892/ijmm.2019.4123

Citation 06

https://www.sciencedirect.com/science/article/pii/S0895398819300455

Citation 07

https://www.spandidos-publications.com/10.3892/mmr.2016.5535?text=abstract

Inter CV

8.1%

Intra CV

5.2%

Detection Instrument

Spectrophotometer

Available Sizes

Frequently Asked Questions

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