PDGF-B (L48) polyclonal antibody

PDGF is a mitogen for mesenchyme- and glia-derived cells. It consists of two disulfide-bonded polypeptide chains, A and B, and occurs as three isoforms, PDGF AA, PDGF AB and PDGF BB. The three isoforms bind with different affinities to two receptor types, α and β, which are structurally related and endowed with protein-tyrosine kinase domains. Ligand binding induces activation of the receptor kinases by formation of receptor dimers; the A subunit of PDGF binds only to α receptors with high affinity, whereas the B subunit can bind to both α and β receptors. Evidence suggests that PDGF may function as a neurotrophic factor.

Platelet-derived growth factor subunit B; PDGF subunit B; PDGF-2; Platelet-derived growth factor B chain; Platelet-derived growth factor beta polypeptide; Proto-oncogene c-Sis; Becaplermin; PDGFB; PDGF2; SIS

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of PDGF-B (L48) pAb at 1:500 dilution Lane1:HepG2 whole cell lysate (40ug) Lane2:3T3-L1 whole cell lysate (40ug) Lane3:H9C2 whole cell lysate (40ug) Lane4:H1792 whole cell lysate (40ug) Lane5:Hela whole cell lysate (40ug) Lane6:HEK293T whole cell lysate (40ug)